NASC

NASC ID: N850334

Name: WiscDsLox289_292E10

ABRC stock number: CS850334

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox289_292E10.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51390.1 (chr 3 pos 19077770 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797473.

Wiscseq_DsLox289_292E10.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G27040.2-P355 and AT2G27050.1-P355 (chr 2 pos 11542972 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797472.

Wiscseq_DsLox289_292E10.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G14940.1 (at chr 4 pos 8544267 (C/8544267-8544346) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 2, C 7 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452095.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox289_292E10

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox289_292E10	WiscDsLox289_292E10	AT2G27040 AT2G27050 AT4G14940 At3g51390

EMBL accession:

CW800859